References

Andersen, C.B.F., Torvund-Jensen , M., Nielsen , M.J., de Oliveira , C.L.P., Hersleth , H.-P., Andersen , N.H., Pedersen , J.S., Andersen , G.R. and Moestrup , S.K. (2012) 'Structure of the haptoglobin–haemoglobin complex ', Nature, no. 489, September, pp. 456-460.

Other Literature:

Alayash, A.I., Andersen, C.B.F., Moestrup, S.K. and Bülow, L. (2013) 'Haptoglobin: the hemoglobin detoxifier in plasma', Cell, no. 31, September, pp. 2-3.

Fabriek, B.O., Dijkstra, C.D. and van den Berg, T.K. (2005) 'The macrophage scavenger receptor CD163', Immunobiology, vol. 210, no. 2-4, August, pp. 153–160.

Mertens, H.D.T. and Svergun, D.I. (2010) 'Structural characterization of proteins and complexes using small-angle X-ray solution scattering', Journal of Structural Biology, vol. 172, no. 1, October, pp. 128–141.

Lim, S.-K., Kim, H., Lim, S.K., bin Ali, A., Lim, Y.K., Wang, Y., Chong, S.M., Costantini, F. and Baumman, H. (1998) ' Increased Susceptibility in Hp Knockout Mice During Acute Hemolysis', Blood, no. 92, September, pp. 1870-1877.

Nantasenamat, C., Prachayasittikul, V. and Bulow, L. (2013) 'Molecular Modeling of the Human Hemoglobin-Haptoglobin Complex Sheds Light on the Protective Mechanisms of Haptoglobin', PLoS ONE, no. 8, July, p. 1.

Polticelli, F., Bocedi, A., Minervini, G. and Ascenzi, P. (2008) 'Human haptoglobin structure and function – a molecular modelling study', The FEBS Journal, vol. 275, no. 22, November, pp. 5648-5656.

Svistunenko, D.A. (2005) 'Reaction of haem containing proteins and enzymes with hydroperoxides: The radical view', Biochimica et Biophysica Acta (BBA) - Bioenergetics, no. 1707, Feburary, pp. 127–155.

Voet, D. and Voet, J.G. (2011) Biochemistry, Fourth Edition edition, Wiley.

Protein Sequences:
UniProt
Porcine Haptoglobin - Q8SPS7
Porcine Haemoglobin alpha- P01965
Porcine Haemoglobin beta- P02067

Human Haptoglobin - P00738
Human Haemoglobin alpha - P69905
Human Haemoglobin beta - P68871

Tools Used:

PyMOL 1.3

GIF Animator

Clustual

Appendix - Clustual alignment for haemoglobin subunits

Haemoglobin alpha

sp|P01965|HBA_PIG        -VLSAADKANVKAAWGKVGGQAGAHGAEALERMFLGFPTTKTYFPHFNLSHGSDQVKAHG

sp|P69905|HBA_HUMAN      MVLSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHFDLSHGSAQVKGHG

                          *** ***:**********.:** :**********.***********:***** ***.**

sp|P01965|HBA_PIG        QKVADALTKAVGHLDDLPGALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHHPDDFNP

sp|P69905|HBA_HUMAN      KKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPAEFTP

                         :*******:**.*:**:* ********************************** * :*.*

sp|P01965|HBA_PIG        SVHASLDKFLANVSTVLTSKYR

sp|P69905|HBA_HUMAN      AVHASLDKFLASVSTVLTSKYR

                         :**********.**********

Haemoglobin beta

sp|P02067|HBB_PIG        MVHLSAEEKEAVLGLWGKVNVDEVGGEALGRLLVVYPWTQRFFESFGDLSNADAVMGNPK

sp|P68871|HBB_HUMAN      MVHLTPEEKSAVTALWGKVNVDEVGGEALGRLLVVYPWTQRFFESFGDLSTPDAVMGNPK

                         ****: ***.** .************************************. ********

sp|P02067|HBB_PIG        VKAHGKKVLQSFSDGLKHLDNLKGTFAKLSELHCDQLHVDPENFRLLGNVIVVVLARRLG

sp|P68871|HBB_HUMAN      VKAHGKKVLGAFSDGLAHLDNLKGTFATLSELHCDKLHVDPENFRLLGNVLVCVLAHHFG

                         ********* :***** **********.*******:**************:* ***:::*

sp|P02067|HBB_PIG        HDFNPNVQAAFQKVVAGVANALAHKYH

sp|P68871|HBB_HUMAN      KEFTPPVQAAYQKVVAGVANALAHKYH

                         ::*.* ****:****************