Small-angle X-ray Scattering (SACS)
SAXS is a method used to solve the overall shape of biomolecules in the size range of 5-100 kDa. The resolution that can be reached with this method is 1-2 nm. As in X-ray crystallography the scattering of X-rays from a sample are measured, however the sample is not in crystalline state and the scattering only occurs at small angles so that scattering curve can be measured (Figure 11) (Mertens and Svergun, 2010).
SAXS is a method used to solve the overall shape of biomolecules in the size range of 5-100 kDa. The resolution that can be reached with this method is 1-2 nm. As in X-ray crystallography the scattering of X-rays from a sample are measured, however the sample is not in crystalline state and the scattering only occurs at small angles so that scattering curve can be measured (Figure 11) (Mertens and Svergun, 2010).
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Figure 11: Schematic view of the SAXS experiment for detection of the scattering curve (taken from Biosax, 2014).
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A computer calculates a structure model consisting of small spheres by approximating the measured scattering curve to a calculated curve for the model. The X-ray beam for calculation of CD163 binding came from a rotating anode X-ray source (Andersen et al., 2012).
it's great how you link the references to the sources online in case anyone wanted to easily carry out some extra reading.
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